Journal: Antioxidants

Article Title: Role of Sulfide Quinone Oxidoreductase and Supersulfides in Hepatic Ischemia–Reperfusion Injury in Mice

doi: 10.3390/antiox15010094

Figure Lengend Snippet: Liver function and histological changes and level of sulfide quinone oxidoreductase (SQOR) in hepatic ischemia–reperfusion injury in mice. ( a ) Flowchart of the experimental protocols. ( b ) The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) 4 h after reperfusion in mice. ( c ) Representative images of hematoxylin and eosin (H&E) staining 4 h after reperfusion in mice. The scale bars are 50 μm in H&E images. ( d ) Upper part of the figure shows representative Western blotting images of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), sulfide quinone oxidoreductase (SQOR), ethylmalonic encephalopathy 1 (ETHE1), thiosulfate sulfurtransferase (TST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein levels in liver extracts from sham and IRI mice. Lower part of the figure shows differences in CBS/GAPDH, CSE/GAPDH, SQOR/GAPDH, ETHE1/GAPDH, and TST/GAPDH in each group. The data are presented as n = 6 per group. Significant differences between groups were determined using one-way analysis of variance. Data are expressed as the means ± standard deviations, **** p < 0.0001.

Article Snippet: After transfer, the membranes were blocked with Every blocking buffer (Bio-Rad Laboratories, Inc.) and immunoblotted with one of the primary antibodies at 4 °C overnight: mouse anti-GAPDH antibody (1:1000; Cell Signaling Technology, Inc., Danvers, MA, USA, Cat# 5174), mouse anti-CSE antibody (1:1000; Cell Signaling Technology, Cat#19689), mouse anti-CBS antibody (1:1000; Cell Signaling Technology, Cat# 14782S), mouse anti-SQOR antibody (1:500; Cell Signaling Technology, Cat#17256-1-AP), mouse anti-TST antibody (1:1000; Gene Tex Company, Cat# GTX114858), and mouse anti-ETHE1 antibody (1:1000; Proteintech Group, Inc., Cat# 27786-1AP).

Techniques: Staining, Western Blot

Journal: Antioxidants

Article Title: Role of Sulfide Quinone Oxidoreductase and Supersulfides in Hepatic Ischemia–Reperfusion Injury in Mice

doi: 10.3390/antiox15010094

Figure Lengend Snippet: Relative protein levels of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), ethylmalonic encephalopathy 1 (ETHE1), and thiosulfate sulfurtransferase (TST) in the livers of AAV-control and AAV-shSQOR and AAV-shSQOR+Na 2 S 3 mice. Representative Western blotting images of CBS, CSE, ETHE1, TST, and GAPDH protein levels in liver extracts from each group of mice and differences in CBS/GAPDH, CSE/GAPDH, ETHE1/GAPDH, and TST/GAPDH in each group. The data are presented as n = 6 per group. Significant differences between groups were determined using one-way analysis of variance. Data are expressed as the means ± standard deviations, * p < 0.05, *** p < 0.001, and **** p < 0.0001.

Article Snippet: After transfer, the membranes were blocked with Every blocking buffer (Bio-Rad Laboratories, Inc.) and immunoblotted with one of the primary antibodies at 4 °C overnight: mouse anti-GAPDH antibody (1:1000; Cell Signaling Technology, Inc., Danvers, MA, USA, Cat# 5174), mouse anti-CSE antibody (1:1000; Cell Signaling Technology, Cat#19689), mouse anti-CBS antibody (1:1000; Cell Signaling Technology, Cat# 14782S), mouse anti-SQOR antibody (1:500; Cell Signaling Technology, Cat#17256-1-AP), mouse anti-TST antibody (1:1000; Gene Tex Company, Cat# GTX114858), and mouse anti-ETHE1 antibody (1:1000; Proteintech Group, Inc., Cat# 27786-1AP).

Techniques: Control, Western Blot